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	<title>Letters to a Persian Cat &#187; Gun | Letters to a Persian Cat</title>
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		<title>Nocturnal dialog on molecular biology</title>
		<link>http://persian-cat.de/?p=1017</link>
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		<pubDate>Wed, 15 Sep 2010 23:53:40 +0000</pubDate>
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		<description><![CDATA[8:32 p.m. Ghazal my Dear, Have a look at the gel. Are you happy with it ? I promise I did not manipulate anything on it: no make-up, no hair dye, mascara, no plastic surgery. It is real, and therefore it is beautiful (just like you are). M. PS: RNA I&#8221;ll tell you in a moment. Stay tuned !!!! &#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8211;&#8230; <a href="http://persian-cat.de/?p=1017">(more...)</a>]]></description>
				<content:encoded><![CDATA[<p>8:32 p.m.</p>
<p>Ghazal my Dear,<br />
Have a look at the gel. Are you happy with it ?<br />
I promise I did not manipulate anything on it:  no make-up, no hair dye, mascara, no plastic surgery. It is real, and therefore it is beautiful (just like you are).<br />
M.<br />
PS: RNA I&#8221;ll tell you in a moment.  Stay tuned !!!!</p>
<p><img src="http://51005274.de.strato-hosting.eu/cgi-data/weblog_basic/uploads/2010/09/ghazals-pcr-gel-late-night-15092010.jpg" alt="ghazals-pcr-gel-late-night-15092010" title="ghazals-pcr-gel-late-night-15092010" width="374" height="374" class="aligncenter size-full wp-image-1081" /></p>
<p>&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8211;<br />
8:44 p.m.</p>
<p>thank you for the gel!!! very kind of you. still the prop1 exon2 JF1 show a lot of bands.and is a bit weak. and the first three bands being prop1 exon1, i see two bands for each sample? or is it the primer dimers? and on the last samples on the first row (being gpx3 exon1), i see many bands or is it good because of the thick band?<br />
on the second row gpx3 exon3- some extra bands as well. or is it okay? so i can use this for the sequencing tomorrow?</p>
<p>i am looking forward to hear about the rna. this is exciting i have to admit!!!</p>
<p>good luck! i will try to look at my thesis now. so i do something useful as well.</p>
<p>&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8211;<br />
9:48 p.m.</p>
<p>Ghazal, my Dear</p>
<p>So happy to have brain RNA now (one JF1 one FVB, each high concentration).<br />
So sad to sit here alone. Nobody to celebrate with. Its boring alone, even the results are o.k.<br />
<img src="http://51005274.de.strato-hosting.eu/cgi-data/weblog_basic/uploads/2010/09/brain-rna.jpg" alt="brain-rna" title="brain-rna" width="960" height=220" class="aligncenter size-full wp-image-1021" /></p>
<p>&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8211;<br />
9:54 p.m.</p>
<p>michael!!!! yey!!! looks really good! im happy too! you did a great job!! so are we going to kill more mice? and then do the extracation of RNA your way?</p>
<p>thank  you michael, for putting so much time and energy for this project.</p>
<p>you should go home and get some rest.</p>
<p>&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8211;<br />
10:06 p.m.<br />
Ghazal dear,</p>
<p>On 15.09.2010 21:54, Ghazal F wrote:<br />
> michael!!!! yey!!! looks really good! im happy too! you did a great job!! so are we going to kill more mice?</p>
<p>I already have thyroids from two mice each strain. I start now with the extraction. Hope of equally good results. Lets see.</p>
<p>> and then do the extracation of RNA your way?</p>
<p>Its not my way. Its the classical way (it stinks, it is more time consuming, but you see at the end works more robust).</p>
<p>><br />
> thank  you michael, for putting so much time and energy for this project.</p>
<p>Its our project. Don&#8221;t forget what you put in it:  5 month lonely life , the people you love so far away. Nobody here to kiss you before going to bed.</p>
<p>><br />
> you should go home and get some rest.</p>
<p>Where is this, home ?</p>
<p>Take Care, my dear (and don&#8221;t you walk around on empty streets in the darkness any more).<br />
Ghazal, I got mad if I think that something happens to you. You remember you talked about Sweden and<br />
that raping happens there more often. Thats in fact one of the main reasons I always got a shock when I hear or read that you are going back.</p>
<p>Michael</p>
<p>&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8211;<br />
10:56 p.m.</p>
<p>> I already have thyroids from two mice each strain. I start now with the<br />
> extraction. Hope of equally good results. Lets see.<br />
michael, we can continue tomorrow?<br />
you are doing too much. i also want to be done with the labwork this week but dont want you to do all this and stay for  so long in the lab.</p>
<p>> Where is this, home ?<br />
your home, where your family is and your bed:)</p>
<p>> Take Care, my dear (and don&#8221;t you walk around on empty streets in the<br />
> darkness any more).  Ghazal, I got mad if I think that something happens to you. You remember<br />
> you talked about Sweden and that raping happens there more often. Thats in fact one of the main<br />
> reasons I always got a shock when I hear or read  that you are going back.</p>
<p>i did not walk in the night,. it was light. i am scared to walk alone at night. and yes, i find munich as a safe place. safer than sweden. i need to walk sometimes, instead of taking bike i walk sometimes, its nice. nice feeling when you walk alone with your own thoughts.</p>
<p>hope u are doing well in lab and that u are going home soon&#8230;<br />
&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8211;<br />
10:51 p.m.<br />
On 15.09.2010 21:54, Ghazal F wrote:</p>
<p>> michael!!!! yey!!! looks really good! im happy too! you did a great job!! so are we going to kill more mice?<br />
Who is going to kill mice ?  You ?   Maybe you first practise this on me. But be aware:  You can kill me with your gun, but not the mice. If you hold a gun on me, I wont move away, cause I&#8221;ll be struck again by the glance of your eyes. Not so the mice:  They can smell the danger and they run away !!</p>
<p>Ghazal, I got the feeling we can speak open to each other, can&#8221;t we ?</p>
<p>TAKE CARE</p>
<p>Michael<br />
&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;-<br />
10:58 p.m.<br />
noooo i am not killing anyone.<br />
yes we can talk open.. or how do you mean<br />
&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8211;<br />
11:04 p.m.<br />
Ghazal my Dear,<br />
Just let me start the Reverse Transcription. (I do it in a PCR cycler, can be programmed to do it and after 1 hour cooles down automatically).<br />
Then you have the cDNA tomorrow morning and can start with PCR right away.</p>
<p>On 15.09.2010 22:58, Ghazal F wrote:<br />
> noooo i am not killing anyone.<br />
> yes we can talk open.. or how do you mean</p>
<p>I&#8221;ll come around and tell you.</p>
<p>&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8211;<br />
11:09 p.m.<br />
come around? i will sleep now also my flatmate is sleeping. so see you tomorrow. thank you again for the cdna!!!!<br />
&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8211;<br />
11:24 p.m.</p>
<p>On 15.09.2010 23:09, Ghazal F wrotei:<br />
> come around? i will sleep now also my flatmate is sleeping.<br />
I&#8221;m not going to wake up your flatmate !!<br />
And you said you are going to work on your thesis the whole night through ?<br />
Did the thesis made you already such tired ?</p>
<p>> so see you tomorrow. thank you again for the cdna!!!!<br />
This, of course was very, very open !!!<br />
And this is what I like you for so much.</p>
<p>Then good night, funny dreams, and at least you can imagine that I kissed you before going to bed.</p>
<p>Take Care</p>
<p>Michael<br />
&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;-<br />
0:00 a.m.<br />
Ghazal my dear,<br />
Are you really already asleep ?   This was a fast thesis writing !!<br />
Hope I haven&#8221;t scared you.</p>
<p>Sleep well<br />
Michael<br />
&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8211;<br />
0:04 a.m.<br />
was trying to sleep. when i saw your email of what you expected of me, to write the thesis, i couldnt sleep. will try to read a bit more now&#8230;</p>
<p>good night<br />
&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8211;<br />
0:11 a.m.</p>
<p>Ghazal my Dear, please no no, shame on me, I didn&#8221;t ment it.<br />
You do everything right. The night is yours. You work the whole day already. Please stop to spoil the best hours of the day with the thesis now. Sleep, please.<br />
I&#8221;m also almost done. Don&#8221;t worry, I won&#8221;t pass by. Maybe another time, not today. It would be silly to first drive away, just to come back later.</p>
<p>I like you anyhow. I will always do.</p>
<p>Michael<br />
&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8211;<br />
0:20 a.m.<br />
if you get hungry i have a sandwich in the fridge.<br />
hope u will  be done soon so u can go home..</p>
<p>good night</p>
<p>&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8211;<br />
0:44 a.m.<br />
Ghazal my Dear,</p>
<p>Sorry for the assumption that you could kill me. Your last e-mail shows how much you care about me.<br />
I found one sandwich. Hope it is the one that you offered: It is brownish, with sliced boiled eggs on it and some letuce, right.</p>
<p>Please tell me it is the right one, it looks delicious.</p>
<p>Something I hope you are delighted (just for your intellectual satisfaction) is the next RNAs: You see they are all above 1µg and ration around 1.8.</p>
<p><img src="http://51005274.de.strato-hosting.eu/cgi-data/weblog_basic/uploads/2010/09/thyroid-brain-rna.jpg" alt="thyroid-brain-rna" title="thyroid-brain-rna" width="960" height="220" class="aligncenter size-full wp-image-1025" /></p>
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		<title>If you can&#8221;t love him, shot him !</title>
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		<pubDate>Tue, 14 Sep 2010 23:05:53 +0000</pubDate>
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		<description><![CDATA[Ghazal my Dear, Tonight I have something very unusual to tell you. It is so unusual, because it is something commercial, about shopping and beeing happy to have found something very useful. I know you got excited now, don&#8221;t you ? You can&#8221;t stop reading any more, right ? You wan&#8221;t to know what it is, don&#8221;t you ? (You&#8230; <a href="http://persian-cat.de/?p=999">(more...)</a>]]></description>
				<content:encoded><![CDATA[<p>Ghazal my Dear,</p>
<p>Tonight I have something very unusual to tell you. It is so unusual, because it is something commercial, about shopping and beeing happy to have found something very useful.</p>
<p>I know you got excited now, don&#8221;t you ?<br />
You can&#8221;t stop reading any more, right ?<br />
You wan&#8221;t to know what it is, don&#8221;t you ?</p>
<p>(You are really sweet !!)</p>
<p>But be aware:  it again has something to do with you and me !! (just a little material and technical aspekt in addition to all the happiness that you brought to me by your shear presence).</p>
<p>It is about the little Smartphone-Adapter-Plug that somehow broke<br />
(Could be that it broke because you poked and bended and cut it, since you thought it is a piece of your fingernail ?) It is not so important, how it broke and why, it is just the adapter, its wasn&#8221;t the Smartphone&#8221;s heart that broke, I know you wouldn&#8221;t be able to do this, you are too much an angel to break hearts.</p>
<p>Well, I searched this special Hewlett Packard Smartphone shop today and guess what:<br />
I found the adapter (same colour:  black, as you prefer for men. No pink. If there would be a pink one, I would have bought both of them: one for me and a pink one for you in case you need the GPS again).</p>
<p>And what is so amazing (and for this I have to be grateful to you, since I would have never found this):<br />
This little adapter thing comes together with a SOLAR ENERGY RECHARGER !!!!<br />
This means, I can not only mount the Smartphone to the car again, but now I can recharge it in the sun !!!</p>
<p>It will always remind me of this summer 2010, which in my memories will always remain as the most sunniest summer ever.<br />
Although the nights in the moon-light and under the meteor showers and the stars were amazing as well. Who knows, maybe the light of the stars and of the moon is sufficient to recharge the phone with this little crazy adaptor.</p>
<p>Ghazal, my dear, tell me what you need to recharge your life battery !<br />
You are always so calm, sometimes I&#8221;d like to make you explode, to beat me in the face, to shout on me, to see you holding a gun on me (of course only with bullets that hurt, but don&#8221;t kill), only to see that you are full of energy.</p>
<p>But this probably would change everything, you would not be you anymore, and for me the illusion, that you are the grown up daughter of the persian family that I met 20 years ago in Berlin would fall down in pieces.</p>
<p>Are you o.k., happy (I know, its hard to tell. One always could imagine beeing a little bit happier).<br />
I permanently feel guilty, sometimes feel as if I have taken you as hostage here in Munich, keeping you in this single room in the guesthouse like a nun in an eremitage.<br />
I only feed you with words, but they can not get rid of your loneliness.<br />
At least I should have given you a cat, such one as Holli Golightly had in &#8220;Breakfast at Tiffany&#8221;s&#8221;. It could lean on you in the evening, and purr if you crawl its fur. It would jump on you lap, when you watch a movie or listen to some music.</p>
<p>Wish you a pleasant night, funny dreams, sleep well</p>
<p>Michael<br />
&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;</p>
<p>michael,</p>
<p>im glad you found a better adapter for your phone! didnt know that such thing exsist, an adapter that charges the phone with sun. interesting. <img src='http://persian-cat.de/wp-includes/images/smilies/icon_smile.gif' alt=':)' class='wp-smiley' /> </p>
<p>haha i dont want to hold a gun against you and shoot, i will stay calm.think it is better. dont think my mum thinks im calm at home.</p>
<p>dont worry about me. i enjoy unterschleissheim. the guesthouse. i feel so relaxed and calm when i am here. and i am so happy with everything.<br />
cats are cute, but only when they are babies.</p>
<p>see you tomorrow</p>
<p>/ghazal</p>
<p>&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;..</p>
<p>See you tomorrow, my Dear.<br />
Thanks to confirm that you enjoy and relax. Even if you would have told me this already a million times: I&#8221;d like to hear it once again !</p>
<p>Sleep well, dream something happy.</p>
<p>Michael</p>
<p>PS:  Ghazal, my Dear, Can you imagine the head-lines in the newspaper:<br />
&#8220;MSc student shoots down its supervisor&#8221;. This would make us sooooo famous.</p>
<p><img src="http://51005274.de.strato-hosting.eu/cgi-data/weblog_basic/uploads/2010/09/daily-news.jpg" alt="daily-news" title="daily-news" width="480" height="520" class="aligncenter size-full wp-image-1010" /></p>
<p>Anyway, have a sweet night, but this time I think I have to take care :-}</p>
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